Immunohistochemical studies on hemoglobin-haptoglobin and hemoglobin catabolism sites.

Maximal erythrocyte and hemoglobin catabolism.

A series of experiments were performed on anesthetized dogs in which varying quantities of normal canine erythrocytes damaged by incubation with N-ethylmaleimide were injected into the circulation. Cell sequestration and catabolism of hemoglobin to carbon monoxide remained normal after injections that contained from 0.058 to 0.154 g of hemoglobin per kg of body weight. After administration of l...

Interaction between hemoglobin subunits in the hemoglobin . haptoglobin complex.

The accessibility of hemoglobin sulfhydryl groups to modification by p-mercuribenzoate @"B) was tested in order to investigate the effect of the hemoglobinhaptoglobin association on the contact between a and /3 chain subunits. Previous evidence indicates that a molecule of tetrameric hemoglobin binds to haptoglobin as two separate a& dimers. Modification of the hemoglobin a and B chain sullhydr...

Studies on the serum haptoglobin level in hemoglobinemia and its influence on renal excretion of hemoglobin.

JAYLI:’ foiiiid that oti addition of a si ttI1 ai ount of hen og1ohin, rtiin shows weak peroxidase a(tivity with au optiintii around pH 4.4 in sJ)itP of the fact that no 50(11 a(1ivitv ean })e dei oiistiated in either suI)stan(e hv itS( 1f. In later investigations iii association with POlOliOVski he (lemonstrated that a complex l)et\VN9I hemoglobin (Jib) an(l a serum protein is responsible for ...

Studies on Hemoglobin Biosynthesis: Asynchronous Synthesis of Hemoglobin a and Hemoglobin A2 by Erythrocyte Precursors.

Heterotropic Effect of β-lactam Antibiotics on Antioxidant Property of Haptoglobin) 2-2(-Hemoglobin Complex

Haptoglobin (Hp) is a mammalian serum glycoprotein showing a genetic polymorphism with three types, 1-1, 2-2 and 1-2. Hp appears to conserve the recycling of heme-iron by forming an essentially irreversible but non-covalent complex with hemoglobin which is released into the plasma by erythrocyte lysis. As an important consequence, Haptoglobin-Hemoglobin complex (Hp-Hb) shows considerable antiox...